TY  - JOUR
A1  - Bolz, Mathias
A1  - Thomas, Laura
A1  - Scheffer, Ute
A1  - Kalden, Elisabeth
A1  - Hartmann, Roland K.
A1  - Göbel, Michael
T1  - Redirection of miRNA‐argonaute complexes to specific target sites by synthetic adaptor molecules
T2  - Chemistry & biodiversity
N2  - Dysregulation of miRNAs is connected with a multitude of diseases for which antagomirs and miRNA replacement are discussed as therapeutic options. Here, we suggest an alternative concept based on the redirection of RISCs to non‐native target sites. Metabolically stable DNA‐LNA mixmers are used to mediate the binding of RISCs to mRNAs without any direct base complementarity to the presented guide RNA strand. Physical redirection of a dye‐labeled miRNA model and of specific miRNA‐programmed RISC fractions present in HeLa extracts is demonstrated by pull‐down experiments with biotinylated capture oligonucleotides.
KW  - argonaute protein
KW  - locked nucleic acids
KW  - RNA induced silencing complex
KW  - pull-down
KW  - Western blot
KW  - mRNA
KW  - RNA recognition
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56514
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-565141
SN  - 1612-1880
VL  - 17
IS  - 7, e2000272
PB  - Wiley-VHCA AG
CY  - Zürich
ER  -