TY  - JOUR
A1  - Widera, Darius
A1  - Klenke, Christin
A1  - Nair, Deepak
A1  - Heidbreder, Meike
A1  - Malkusch, Sebastian
A1  - Sibarita, Jean-Baptiste
A1  - Choquet, Daniel
A1  - Kaltschmidt, Barbara
A1  - Heilemann, Mike
A1  - Kaltschmidt, Christian
T1  - Single-particle tracking uncovers dynamics of glutamate-induced retrograde transport of NF-κB p65 in living neurons
T2  - Neurophotonics
N2  - Retrograde transport of NF-κB from the synapse to the nucleus in neurons is mediated by the dynein/dynactin motor complex and can be triggered by synaptic activation. The caliber of axons is highly variable ranging down to 100 nm, aggravating the investigation of transport processes in neurites of living neurons using conventional light microscopy. We quantified for the first time the transport of the NF-κB subunit p65 using high-density single-particle tracking in combination with photoactivatable fluorescent proteins in living mouse hippocampal neurons. We detected an increase of the mean diffusion coefficient (Dmean) in neurites from 0.12±0.05 to 0.61±0.03  μm2/s after stimulation with glutamate. We further observed that the relative amount of retrogradely transported p65 molecules is increased after stimulation. Glutamate treatment resulted in an increase of the mean retrograde velocity from 10.9±1.9 to 15±4.9  μm/s, whereas a velocity increase from 9±1.3 to 14±3  μm/s was observed for anterogradely transported p65. This study demonstrates for the first time that glutamate stimulation leads to an increased mobility of single NF-κB p65 molecules in neurites of living hippocampal neurons.
KW  - retrograde transport
KW  - single-particle tracking with photoactivated-localization microscopy
KW  - single molecule
KW  - NF-kappaB
KW  - neurons
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50481
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-504810
SN  - 2329-4248
SN  - 2329-423X
N1  - © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
VL  - 3
IS  - 4, Art. 041804
SP  - 041804-1
EP  - 041804-6
PB  - SPIE
CY  - Bellingham, Wash.
ER  -