TY  - JOUR
A1  - Weber, Birte
A1  - Lackner, Ina
A1  - Baur, Meike
A1  - Fois, Giorgio
A1  - Gebhard, Florian
A1  - Marzi, Ingo
A1  - Schrezenmeier, Hubert
A1  - Relja, Borna
A1  - Kalbitz, Miriam
T1  - Effects of circulating HMGB-1 and histones on cardiomyocytes−hemadsorption of these DAMPs as therapeutic strategy after multiple trauma
T2  - Journal of clinical medicine
N2  - Background and purpose: The aim of the study was to determine the effects of post-traumatically released High Mobility Group Box-1 protein (HMGB1) and extracellular histones on cardiomyocytes (CM). We also evaluated a therapeutic option to capture circulating histones after trauma, using a hemadsorption filter to treat CM dysfunction. Experimental Approach: We evaluated cell viability, calcium handling and mitochondrial respiration of human cardiomyocytes in the presence of HMGB-1 and extracellular histones. In a translational approach, a hemadsorption filter was applied to either directly eliminate extracellular histones or to remove them from blood samples obtained from multiple injured patients. Key results: Incubation of human CM with HMGB-1 or histones is associated with changes in calcium handling, a reduction of cell viability and a substantial reduction of the mitochondrial respiratory capacity. Filtrating plasma from injured patients with a hemadsorption filter reduces histone concentration ex vivo and in vitro, depending on dosage. Conclusion and implications: Danger associated molecular patterns such as HMGB-1 and extracellular histones impair human CM in vitro. A hemadsorption filter could be a therapeutic option to reduce high concentrations of histones.
KW  - hemadsorption
KW  - early myocardial damage (EMD)
KW  - DAMPs
KW  - post-traumatic
KW  - calcium handling
KW  - mitochondrial dysfunction
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54814
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-548140
SN  - 2077-0383
VL  - 9
IS  - 1421
PB  - MDPI
CY  - Ba
ER  -