TY  - JOUR
A1  - Bhavsar, Mit Balvantray
A1  - Cato, Gloria
A1  - Hauschild, Alexander
A1  - Leppik, Liudmila
A1  - Costa Oliveira, Karla Mychellyne
A1  - Eischen-Loges, Maria José
A1  - Barker, John Howard
T1  - Membrane potential (Vmem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation
T2  - PeerJ
N2  - Background: Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation, differentiation and migration, all playing a critical role in embryonic development, healing, and regeneration.
Methods: With the goal of analyzing the changes in Vmem during cell proliferation and differentiation, here we used direct current electrical stimulation (EStim) to promote cell proliferation and differentiation and simultaneously tracked the corresponding changes in Vmem in adipose derived mesenchymal stem cells (AT-MSC).
Results: We found that EStim caused increased AT-MSC proliferation that corresponded to Vmem depolarization and increased osteogenic differentiation that corresponded to Vmem hyperpolarization. Taken together, this shows that Vmem changes associated with EStim induced cell proliferation and differentiation can be accurately tracked during these important cell functions. Using this tool to monitor Vmem changes associated with these important cell behaviors we hope to learn more about how these electrochemical cues regulate cell function with the ultimate goal of developing new EStim based treatments capable of controlling healing and regeneration.
KW  - Membrane potential
KW  - Electrical stimulation
KW  - Mesenchymal stem cells
KW  - Osteogenic differentiation
KW  - Cell proliferation
KW  - Vmem
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/49067
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-490677
SN  - 2167-8359
N1  - Distributed under Creative Commons CC-BY 4.0
VL  - 7
IS  - e6341
SP  - 1
EP  - 13
PB  - PeerJ, Inc.
CY  - London [u. a.]
ER  -