TY  - JOUR
A1  - Peetz, Oliver
A1  - Hellwig, Nils
A1  - Henrich, Erik
A1  - Mezhyrova, Julija
A1  - Dötsch, Volker
A1  - Bernhard, Frank
A1  - Morgner, Nina
T1  - LILBID and nESI : different native mass spectrometry techniques as tools in structural biology
T2  - Journal of the American Society for Mass Spectrometry
N2  - Native mass spectrometry is applied for the investigation of proteins and protein complexes worldwide. The challenge in native mass spectrometry is maintaining the features of the proteins of interest, such as oligomeric state, bound ligands, or the conformation of the protein complex, during transfer from solution to gas phase. This is an essential prerequisite to allow conclusions about the solution state protein complex, based on the gas phase measurements. Therefore, soft ionization techniques are required. Widely used for the analysis of protein complexes are nanoelectro spray ionization (nESI) mass spectrometers. A newer ionization method is laser induced liquid bead ion desorption (LILBID), which is based on the release of protein complexes from solution phase via infrared (IR) laser desorption. We use both methods in our lab, depending on the requirements of the biological system we are interested in. Here we benchmark the performance of our LILBID mass spectrometer in comparison to a nESI instrument, regarding sample conditions, buffer and additive tolerances, dissociation mechanism and applicability towards soluble and membrane protein complexes.
KW  - Native mass spectrometry
KW  - LILBID
KW  - nESI
KW  - Soluble proteins
KW  - Membrane proteins
KW  - Ion source
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50139
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-501393
SN  - 1879-1123
SN  - 1044-0305
N1  - Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
N1  - Correction erschienen in: Journal of The American Society for Mass Spectrometry, 30.2019, Nr. 3, S. 561
VL  - 30
IS  - 1
SP  - 181
EP  - 191
PB  - Springer
CY  - New York [u. a.]
ER  -