TY  - JOUR
A1  - Tamura, Naoka
A1  - Simon, Judith E.
A1  - Nayak, Arnab
A1  - Shenoy, Rajesh
A1  - Hiroi, Noriko
A1  - Boilot, Viviane
A1  - Funahashi, Akira
A1  - Draviam, Viji M.
T1  - A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset
T2  - Biology open
N2  - Microtubules execute diverse mitotic events that are spatially and temporally separated; the underlying regulation is poorly understood. By combining drug treatments, large-scale immunoprecipitation and mass spectrometry, we report the first comprehensive map of mitotic phase-specific protein interactions of the microtubule-end binding protein, EB1. EB1 interacts with some, but not all, of its partners throughout mitosis. We show that the interaction of EB1 with Astrin-SKAP complex, a key regulator of chromosome segregation, is enhanced during prometaphase, compared to anaphase. We find that EB1 and EB3, another EB family member, can interact directly with SKAP, in an SXIP-motif dependent manner. Using an SXIP defective mutant that cannot interact with EB, we uncover two distinct pools of SKAP at spindle microtubules and kinetochores. We demonstrate the importance of SKAP's SXIP-motif in controlling microtubule growth rates and anaphase onset, without grossly disrupting spindle function. Thus, we provide the first comprehensive map of temporal changes in EB1 interactors during mitosis and highlight the importance of EB protein interactions in ensuring normal mitosis.
KW  - Cell cortex
KW  - Kinetochore
KW  - Microtubule
KW  - Mitosis
KW  - Plus-end
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/36933
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-369337
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4365484
SN  - 2046-6390
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
VL  - 4
IS  - 2
SP  - 155
EP  - 169
PB  - Company of Biologists
CY  - Cambridge
ER  -