TY  - JOUR
A1  - Bauer, Roland
A1  - Lauer, Regina
A1  - Linz, Birgit
A1  - Pittner, Fritz
A1  - Peschek, Günter A.
A1  - Ecker, Gerhard F.
A1  - Friedl, Peter
A1  - Noe, Christian R.
T1  - An in vitro model for blood brain barrier permeation
T2  - Scientia pharmaceutica
N2  - The ability to permeate accross the blood brain barrier (BBB) is essential for drugs acting on the central nervous system (CNS). Thus, systems that allow rapid and inexpensive screening of the BBB-permeability properties of novel lead compounds are of great importance for speeding up the drug discovery process in the CNS-area. We used immortalized porcine brain microvessel endothelial cells (PBMECICl-2) to develop a model for measurement of blood-brain barrier permeation of CNS active drugs. Investigation of different cell culture conditions showed, that a system using C6 astrocyte glioma conditioned medium and addition of a cyclic AMP analog in combination with a type IV phosphodiesterase inhibitor (R020-1724) leads to cell layers with transendothelial electrical resistance values up to 300 Ω.cm2. Permeability studies with U-[14C]sucroseg ave a permeability coefficient Pe of 3.24 + 0.14 × 10−4 cm/min, which is in good agreement to published values and thus indicates the formation of tight junctions in vitro.
KW  - Blood brain barrier
KW  - in vitro model
KW  - immortalized porcine brain microvascular endothelial cells
Y1  - 2002
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54368
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-543682
SN  - 2218-0532
SN  - 0036-8709
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 70
IS  - 4
SP  - 317
EP  - 322
PB  - Österr. Apotheker-Verl.-Ges.
CY  - Wien
ER  -