TY  - JOUR
A1  - Altınçekiç, Nadide
A1  - Korn, Sophie Marianne
A1  - Qureshi, Nusrat Shahin
A1  - Dujardin, Marie
A1  - Ninot-Pedrosa, Martí
A1  - Abele, Rupert
A1  - Abi Saad, Marie Jose
A1  - Alfano, Caterina
A1  - Almeida, Fabio C. L.
A1  - Alshamleh, Islam
A1  - Amorim, Gisele Cardoso de
A1  - Anderson, Thomas K.
A1  - Anobom, Cristiane D.
A1  - Anorma, Chelsea
A1  - Bains, Jasleen Kaur
A1  - Bax, Adriaan
A1  - Blackledge, Martin
A1  - Blechar, Julius
A1  - Böckmann, Anja
A1  - Brigandat, Louis
A1  - Bula, Anna
A1  - Bütikofer, Matthias
A1  - Camacho-Zarco, Aldo R.
A1  - Carlomagno, Teresa
A1  - Caruso, Icaro Putinhon
A1  - Ceylan, Betül
A1  - Chaikuad, Apirat
A1  - Chu, Feixia
A1  - Cole, Laura
A1  - Crosby, Marquise G.
A1  - De Jesus, Vanessa
A1  - Dhamotharan, Karthikeyan
A1  - Felli, Isabella C.
A1  - Ferner, Jan
A1  - Fleischmann, Yanick
A1  - Fogeron, Marie-Laure
A1  - Fourkiotis, Nikolaos K.
A1  - Fuks, Christin
A1  - Fürtig, Boris
A1  - Gallo, Angelo
A1  - Gande, Santosh L.
A1  - Gerez, Juan Atilio
A1  - Ghosh, Dhiman
A1  - Gomes-Neto, Francisco
A1  - Gorbatyuk, Oksana
A1  - Guseva, Serafima
A1  - Hacker, Carolin
A1  - Häfner, Sabine
A1  - Hao, Bing
A1  - Hargittay, Bruno
A1  - Henzler-Wildman, Katherine
A1  - Hoch, Jeffrey C.
A1  - Hohmann, Katharina Felicitas
A1  - Hutchison, Marie T.
A1  - Jaudzems, Kristaps
A1  - Jović, Katarina
A1  - Kaderli, Janina
A1  - Kalnins, Gints
A1  - Kanepe, Iveta
A1  - Kirchdoerfer, Robert N.
A1  - Kirkpatrick, John
A1  - Knapp, Stefan
A1  - Krishnathas, Robin
A1  - Kutz, Felicitas
A1  - Zur Lage, Susanne
A1  - Lambertz, Roderick
A1  - Lang, Andras
A1  - Laurents, Douglas
A1  - Lecoq, Lauriane
A1  - Linhard, Verena
A1  - Löhr, Frank
A1  - Malki, Anas
A1  - Bessa, Luiza Mamigonian
A1  - Martin, Rachel W.
A1  - Matzel, Tobias
A1  - Maurin, Damien
A1  - McNutt, Seth W.
A1  - Mebus-Antunes, Nathane Cunha
A1  - Meier, Beat H.
A1  - Meiser, Nathalie
A1  - Mompeán, Miguel
A1  - Monaca, Elisa
A1  - Montserret, Roland
A1  - Perez, Laura Mariño
A1  - Moser, Celine
A1  - Muhle-Goll, Claudia
A1  - Neves-Martins, Thais Cristtina
A1  - Ni, Xiamonin
A1  - Norton-Baker, Brenna
A1  - Pierattelli, Roberta
A1  - Pontoriero, Letizia
A1  - Pustovalova, Yulia
A1  - Ohlenschläger, Oliver
A1  - Orts, Julien
A1  - Poian, Andrea T. da
A1  - Pyper, Dennis Joshua
A1  - Richter, Christian
A1  - Riek, Roland
A1  - Rienstra, Chad M.
A1  - Robertson, Angus
A1  - Pinheiro, Anderson S.
A1  - Sabbatella, Raffaele
A1  - Salvi, Nicola
A1  - Saxena, Krishna
A1  - Schulte, Linda
A1  - Schiavina, Marco
A1  - Schwalbe, Harald
A1  - Silber, Mara
A1  - Almeida, Marcius da Silva
A1  - Sprague-Piercy, Marc A.
A1  - Spyroulias, Georgios A.
A1  - Sreeramulu, Sridhar
A1  - Tants, Jan-Niklas
A1  - Tars, Kaspars
A1  - Torres, Felix
A1  - Töws, Sabrina
A1  - Trevino, Miguel A.
A1  - Trucks, Sven
A1  - Tsika, Aikaterini C.
A1  - Varga, Krisztina
A1  - Wang, Ying
A1  - Weber, Marco E.
A1  - Weigand, Julia E.
A1  - Wiedemann, Christoph
A1  - Wirmer-Bartoschek, Julia
A1  - Wirtz Martin, Maria Alexandra
A1  - Zehnder, Johannes
A1  - Hengesbach, Martin
A1  - Schlundt, Andreas
T1  - Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications
T2  - Frontiers in molecular biosciences
N2  - The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
KW  - COVID-19
KW  - SARS-CoV-2
KW  - nonstructural proteins
KW  - structural proteins
KW  - accessory proteins
KW  - intrinsically disordered region
KW  - cell-free protein synthesis
KW  - NMR spectroscopy
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/61048
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-610488
SN  - 2296-889X
N1  - This work was supported by Goethe University (Corona funds), the DFG-funded CRC: “Molecular Principles of RNA-Based Regulation,” DFG infrastructure funds (project numbers: 277478796, 277479031, 392682309, 452632086, 70653611), the state of Hesse (BMRZ), the Fondazione CR Firenze (CERM), and the IWB-EFRE-program 20007375. This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 871037. AS is supported by DFG Grant SCHL 2062/2-1 and by the JQYA at Goethe through project number 2019/AS01. Work in the lab of KV was supported by a CoRE grant from the University of New Hampshire. The FLI is a member of the Leibniz Association (WGL) and financially supported by the Federal Government of Germany and the State of Thuringia. Work in the lab of RM was supported by NIH (2R01EY021514) and NSF (DMR-2002837). BN-B was supported by the NSF GRFP. MC was supported by NIH (R25 GM055246 MBRS IMSD), and MS-P was supported by the HHMI Gilliam Fellowship. Work in the labs of KJ and KT was supported by Latvian Council of Science Grant No. VPP-COVID 2020/1-0014. Work in the UPAT’s lab was supported by the INSPIRED (MIS 5002550) project, which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and cofinanced by Greece and the EU (European Regional Development Fund) and the FP7 REGPOT CT-2011-285950–“SEE-DRUG” project (purchase of UPAT’s 700 MHz NMR equipment). Work in the CM-G lab was supported by the Helmholtz society. Work in the lab of ABö was supported by the CNRS, the French National Research Agency (ANR, NMR-SCoV2- ORF8), the Fondation de la Recherche Médicale (FRM, NMR-SCoV2-ORF8), and the IR-RMN-THC Fr3050 CNRS. Work in the lab of BM was supported by the Swiss National Science Foundation (Grant number 200020_188711), the Günthard Stiftung für Physikalische Chemie, and the ETH Zurich. Work in the labs of ABö and BM was supported by a common grant from SNF (grant 31CA30_196256). This work was supported by the ETH Zurich, the grant ETH 40 18 1, and the grant Krebsliga KFS 4903 08 2019. Work in the lab of the IBS Grenoble was supported by the Agence Nationale de Recherche (France) RA-COVID SARS2NUCLEOPROTEIN and European Research Council Advanced Grant DynamicAssemblies. Work in the CA lab was supported by Patto per il Sud della Regione Siciliana–CheMISt grant (CUP G77B17000110001). Part of this work used the platforms of the Grenoble Instruct-ERIC center (ISBG; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). Work at the UW-Madison was supported by grant numbers NSF MCB2031269 and NIH/NIAID AI123498. MM is a Ramón y Cajal Fellow of the Spanish AEI-Ministry of Science and Innovation (RYC2019-026574-I), and a “La Caixa” Foundation (ID 100010434) Junior Leader Fellow (LCR/BQ/PR19/11700003). Funded by project COV20/00764 from the Carlos III Institute of Health and the Spanish Ministry of Science and Innovation to MM and DVL. VDJ was supported by the Boehringer Ingelheim Fonds. Part of this work used the resources of the Italian Center of Instruct-ERIC at the CERM/CIRMMP infrastructure, supported by the Italian Ministry for University and Research (FOE funding). CF was supported by the Stiftung Polytechnische Gesellschaft. Work in the lab of JH was supported by NSF (RAPID 2030601) and NIH (R01GM123249).
VL  - 8
IS  - art. 653148
SP  - 1
EP  - 25
PB  - Frontiers
CY  - Lausanne
ER  -