TY - JOUR A1 - Eickholz, Peter A1 - Asendorf, Anne A1 - Schröder, Mario Pascal A1 - Schacher, Beate A1 - Oremek, Gerhard A1 - Schubert, Ralf A1 - Wohlfeil, Martin A1 - Zuhr, Otto T1 - Effect of subgingival instrumentation on neutrophil elastase and C-reactive protein in grade B and C periodontitis: exploratory analysis of a prospective cohort study T2 - Journal of Clinical Medicine N2 - Background: Assessment of the effect of subgingival instrumentation (SI) on systemic inflammation in periodontitis grades B (BP) and C (CP). Methods: In this prospective cohort study, eight BP and 46 CP patients received SI. Data were collected prior to and 12 weeks after SI. Blood was sampled prior to, one day, 6, and 12 weeks after SI. Neutrophil elastase (NE), C-reactive protein (CRP), leukocyte count, lipopolysaccharide binding protein, interleukin 6 (IL-6) and IL-8 were assessed. Results: Both groups showed significant clinical improvement. NE was lower in BP than CP at baseline and 1 day after SI, while CRP was lower in BP than CP at baseline (p < 0.05). NE and CRP had a peak 1 day after SI (p < 0.05). Between-subjects effects due to CP (p = 0.042) and PISA (p = 0.005) occurred. Within-subjects NE change was confirmed and modulated by grade (p = 0.017), smoking (p = 0.029), number of teeth (p = 0.033), and PISA (p = 0.002). For CRP between-subjects effects due to BMI (p = 0.008) were seen. Within-subjects PISA modulated the change of CRP over time (p = 0.017). Conclusions: In untreated CP, NE and CRP were higher than in BP. SI results in better PPD and PISA reduction in BP than CP. Trial registration: Deutsches Register Klinischer Studien DRKS00026952 28 October 2021 registered retrospectively. KW - neutrophil elastase KW - CRP KW - cytokine(s) KW - non-surgical periodontal therapy KW - periodontal– systemic disease interactions KW - periodontitis Y1 - 2022 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72038 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-720382 SN - 2077-0383 N1 - This study was in part funded by the authors and their institutions and in part by grants of the German Society of Periodontology (DG PARO), the German Society of Dental, Oral, and Maxillofacial Medicine (DGZMK), the New Working Group for Periodontology (NAgP) (3 months examination), and the Freiherr Carl von Rothschild’sche Stiftung Carolinum, Frankfurt, Germany (statistical analysis for NE, CRP, leukocyte count, LPS, IL-6, IL-8). N1 - The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request. VL - 11 IS - 11, art. 3189 SP - 1 EP - 11 PB - MDPI CY - Basel ER -