TY  - JOUR
A1  - Ariyawansa, Hiran A.
A1  - Tsai, Ichen
A1  - Wang, Jian-Yuan
A1  - Withee, Patchareeya
A1  - Tanjira, Medsaii
A1  - Lin, Shiou-Ruei
A1  - Suwannarach, Nakarin
A1  - Kumla, Jaturong
A1  - Elgorban, Abdallah M.
A1  - Cheewangkoon, Ratchadawan
T1  - Molecular phylogenetic diversity and biological characterization of diaporthe species associated with leaf spots of camellia sinensis in Taiwan
T2  - Plants
N2  - Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.
KW  - endophytes
KW  - foliar pathogens
KW  - pathogenicity
KW  - taxonomy
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62680
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-626801
SN  - 2223-7747
N1  - This study was funded by grants from the Higher Education Sprout Projects (grant number: NTUJP-108L7215 and NTUJP-110L7221) and The Researchers Supporting Project number (RSP-2021/56), King Saud University, Riyadh, Saudi Arabia.
VL  - 10
IS  - 7, art. 1434
SP  - 1
EP  - 20
PB  - MDPI
CY  - Basel
ER  -