TY  - JOUR
A1  - Tichy, Julia Frederike
A1  - Zinke, Jenny
A1  - Bunz, Benedikt Burchard Max
A1  - Meyermann, Richard
A1  - Harter, Patrick Nikolaus
A1  - Mittelbronn, Michel Guy André
T1  - Expression profile of sonic hedgehog pathway members in the developing human fetal brain
T2  - BioMed research international
N2  - The Sonic Hedgehog (SHH) pathway plays a central role in the developing mammalian CNS. In our study, we aimed to investigate the spatiotemporal SHH pathway expression pattern in human fetal brains. We analyzed 22 normal fetal brains for Shh, Patched, Smoothened, and Gli1-3 expression by immunohistochemistry. In the telencephalon, strongest expression of Shh, Smoothened, and Gli2 was found in the cortical plate (CP) and ventricular zone. Patched was strongly upregulated in the ventricular zone and Gli1 in the CP. In the cerebellum, SHH pathway members were strongly expressed in the external granular layer (EGL). SHH pathway members significantly decreased over time in the ventricular and subventricular zone and in the cerebellar EGL, while increasing levels were found in more superficial telencephalic layers. Our findings show that SHH pathway members are strongly expressed in areas important for proliferation and differentiation and indicate a temporal expression gradient in telencephalic and cerebellar layers probably due to decreased proliferation of progenitor cells and increased differentiation. Our data about the spatiotemporal expression of SHH pathway members in the developing human brain serves as a base for the understanding of both normal and pathological CNS development.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/39389
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-393893
SN  - 2314-6141
N1  - Copyright © 2015 Julia Tichy  et al. This is an open access article distributed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2015
IS  - Article ID 494269
SP  - 1
EP  - 15
PB  - Hindawi
CY  - New York
ER  -