TY  - JOUR
A1  - Mühleip, Jovana Jasmin
A1  - Lin, Yi-Pin
A1  - Kraiczy, Peter
T1  - Further insights into the interaction of human and animal complement regulator factor H with viable lyme disease spirochetes
T2  - Frontiers in veterinary science
N2  - Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato (s.l.) complex differ in their ability to establish infection and to survive in diverse vertebrate hosts. Association with and adaption to various hosts most likely correlates with the spirochetes' ability to acquire complement regulator factor H (FH) to overcome the host's innate immune response. Here we assessed binding of serum FH from human and various animals including bovine, cat, chicken, dog, horse, mouse, rabbit, and rat to viable B. burgdorferi sensu stricto (s.s.), B. afzelii, B. garinii, B. spielmanii, B. valaisiana, and B. lusitaniae. Spirochetes ectopically producing CspA orthologs of B. burgdorferi s.s., B. afzelii, and B. spielmanii, CspZ, ErpC, and ErpP, respectively, were also investigated. Our comparative analysis using viable bacterial cells revealed a striking heterogeneity among Lyme disease spirochetes regarding their FH-binding patterns that almost mirrors the serum susceptibility of the respective borrelial genospecies. Moreover, native CspA from B. burgdorferi s.s., B. afzelii, and B. spielmanii as well as CspZ were identified as key ligands of FH from human, horse, and rat origin while ErpP appears to bind dog and mouse FH and to a lesser extent human FH. By contrast, ErpC did not bind FH from human as well as from animal origin. These findings indicate a strong restriction of distinct borrelial proteins toward binding of polymorphic FH of various vertebrate hosts.
KW  - Borrelia
KW  - complement
KW  - factor H
KW  - Lyme disease
KW  - immune evasion
KW  - spirochete
KW  - innate immunity
KW  - hostpathogen interaction
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/49086
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-490867
SN  - 2297-1769
N1  - Copyright © 2019 Mühleip, Lin and Kraiczy. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
VL  - 5
IS  - Art. 346
SP  - 1
EP  - 11
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  -