TY  - JOUR
A1  - Bojunga, Jörg
A1  - Röddiger, Sandra Johanna
A1  - Stanisch, Michael
A1  - Kusterer, Klaus
A1  - Kurek, Ralf
A1  - Renneberg, Heiner
A1  - Adams, Stefan
A1  - Lindhorst, Elmar
A1  - Usadel, Klaus-Henning
A1  - Schumm-Draeger, Petra-Maria
T1  - Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR
T2  - British journal of cancer
N2  - The sensitive detection of circulating tumour cells in patients with differentiated thyroid cancer may precede the detection of relapse by other diagnostic studies – such as serum thyroglobulin – and thus may have important therapeutic and prognostic implications. We performed reverse transcription-polymerase chain reaction (RT-PCR) on blood samples from patients diagnosed with thyroid disease using two different RT-PCR sensitivities. Additionally, tissue specificity of TG mRNA-expression was determined using RNA extracts from 27 different human tissues. The lower limit of detection was 50–100 TG mRNA producing cells/ml blood using a ‘normal’ RT-PCR sensitivity and 10–20 cells/ml blood using a ‘high’ sensitivity. With the normal sensitivity TG mRNA was detected in 9/13 patients with thyroid cancer and metastasis, 63/137 patients with a history of thyroid cancer and no metastasis, 21/85 with non-malignant thyroid disease and 9/50 controls. With the high sensitivity TG mRNA was detected in 11/13 patients with thyroid cancer and metastasis, 111/137 patients with a history of thyroid cancer and no metastasis, 61/85 with non-malignant thyroid disease and 41/50 controls. Interestingly, using the normal RT-PCR sensitivity TG mRNA transcripts are specific for thyroid tissue and detectable in the peripheral blood of controls and patients with thyroid disease, which correlates with a diagnosis of metastasized thyroid cancer. However, with a high RT-PCR sensitivity, TG mRNA expression was found not to be specific for thyroid tissue and was not correlated with a diagnosis of thyroid cancer in patients. As a consequence, to date TG mRNA detected by RT-PCR in the peripheral blood cannot be recommended as a tumour marker superior to TG serum-level.
KW  - polymerase chain reaction
KW  - reverse transcription
KW  - thyroid cancer
KW  - thyroglobulin mRNA
KW  - molecular diagnostics
Y1  - 2000
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45763
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-457632
SN  - 1532-1827
SN  - 0007-0920
N1  - From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
VL  - 82
IS  - 10
SP  - 1650
EP  - 1655
PB  - Churchill Livingstone
CY  - Edinburgh
ER  -