TY  - JOUR
A1  - Schrecker, Christopher
A1  - Behrens, Sophia
A1  - Schönherr, Rebecca
A1  - Ackermann, Anne
A1  - Pauli, Daniel
A1  - Plotz, Guido
A1  - Zeuzem, Stefan
A1  - Brieger, Angela
T1  - SPTAN1 expression predicts treatment and survival outcomes in colorectal cancer
T2  - Cancers
N2  - Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality. In a cohort of 189 patients with CRC, we recently showed that expression of the cytoskeletal scaffolding protein non-erythroid spectrin αII (SPTAN1) was lower in advanced metastatic tumours. The aim of the present study was to clarify the association of intratumoural SPTAN1 expression levels with treatment and survival outcomes in patients with CRC. The analysis was based on histologic assessment of SPTAN1 protein levels in our own CRC cohort, and transcriptome data of 573 CRC cases from The Cancer Genome Atlas (TCGA). We first establish that high intratumoural levels of SPTAN1 protein and mRNA associate with favourable survival outcomes in patients with CRC. Next, a response prediction signature applied to the TCGA data reveals a possible link between high SPTAN1 transcript levels and improved patient responses to FOLFOX chemotherapy. Complementary in vitro experiments confirm that SPTAN1 knockdown strains of the colon cancer cell lines HT-29, HCT116 mlh1-2 and Caco-2 are less responsive to FOLFOX chemotherapy compared with SPTAN1-proficient control strains. Taken together, we identify SPTAN1 as a novel prognostic biomarker in CRC and show that SPTAN1 expression levels may predict patient responses to chemotherapy. These investigations illustrate how an affordable, histology-based diagnostic test could directly impact therapeutic decision-making at the bedside.
KW  - colorectal cancer
KW  - SPTAN1
KW  - spectrin
KW  - cytoskeleton
KW  - metastasis
KW  - chemotherapy
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62485
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-624855
SN  - 2072-6694
N1  - This work was supported by the Paul and Ursula Klein Foundation, as well as institutional funds from the University Hospital Frankfurt. Christopher Schrecker was supported by a personal grant from the German Research Foundation (Deutsche Forschungsgemeinschaft). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
VL  - 13
IS  - 14, art. 3638
SP  - 1
EP  - 19
PB  - MDPI
CY  - Basel
ER  -