TY  - JOUR
A1  - Brunauer, Anna
A1  - Verboket, René
A1  - Kainz, Daniel M.
A1  - Stetten, Felix von
A1  - Früh, Susanna Maria
T1  - Rapid detection of pathogens in wound exudate via nucleic acid lateral flow immunoassay
T2  - Biosensors
N2  - The rapid detection of pathogens in infected wounds can significantly improve the clinical outcome. Wound exudate, which can be collected in a non-invasive way, offers an attractive sample material for the detection of pathogens at the point-of-care (POC). Here, we report the development of a nucleic acid lateral flow immunoassay for direct detection of isothermally amplified DNA combined with fast sample preparation. The streamlined protocol was evaluated using human wound exudate spiked with the opportunistic pathogen Pseudomonas aeruginosa that cause severe health issues upon wound colonization. A detection limit of 2.1 × 105 CFU per mL of wound fluid was achieved, and no cross-reaction with other pathogens was observed. Furthermore, we integrated an internal amplification control that excludes false negative results and, in combination with the flow control, ensures the validity of the test result. The paper-based approach with only three simple hands-on steps has a turn-around time of less than 30 min and covers the complete analytical process chain from sample to answer. This newly developed workflow for wound fluid diagnostics has tremendous potential for reliable pathogen POC testing and subsequent target-oriented therapy.
KW  - recombinase polymerase amplification
KW  - nucleic acid lateral flow immunoassay
KW  - point-of-care diagnostics
KW  - paper-based detection
KW  - nucleic acid amplification test
KW  - wound exudate
KW  - wound infection
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62145
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-621453
SN  - 2079-6374
N1  - Funding from the German Research Foundation (DFG), within the project “Hochdynamis- che Multianalyte-Assays” (grant number 397660978), is gratefully acknowledged.
VL  - 11
IS  - 3, art. 74
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  -