TY  - JOUR
A1  - Urban, Johannes H.
A1  - Schneider, Richard M.
A1  - Compte, Marta
A1  - Finger, Carsten
A1  - Cichutek, Klaus
A1  - Álvarez-Vallina, Luis
A1  - Buchholz, Christian
T1  - Selection of functional human antibodies from retroviral display libraries
T2  - Nucleic acids research
N2  - Antibody library technology represents a powerful tool for the discovery and design of antibodies with high affinity and specificity for their targets. To extend the technique to the expression and selection of antibody libraries in an eukaryotic environment, we provide here a proof of concept that retroviruses can be engineered for the display and selection of variable single-chain fragment (scFv) libraries. A retroviral library displaying the repertoire obtained after a single round of selection of a human synthetic scFv phage display library on laminin was generated. For selection, antigen-bound virus was efficiently recovered by an overlay with cells permissive for infection. This approach allowed more than 10(3)-fold enrichment of antigen binders in a single selection cycle. After three selection cycles, several scFvs were recovered showing similar laminin-binding activities but improved expression levels in mammalian cells as compared with a laminin-specific scFv selected by the conventional phage display approach. Thus, translational problems that occur when phage-selected antibodies have to be transferred onto mammalian expression systems to exert their therapeutic potential can be avoided by the use of retroviral display libraries.
Y1  - 2005
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/32464
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-324646
UR  - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC549574/
SN  - 1362-4962
SN  - 0305-1048
N1  - Copyright © The Author 2005. Published by Oxford University Press. All rights reserved The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org
VL  - 33
IS  - 4
SP  - 1
EP  - 8
PB  - Oxford Univ. Press
CY  - Oxford
ER  -