TY  - JOUR
A1  - Hoock, Samira Catharina
A1  - Ritter, Andreas Hans
A1  - Steinhäuser, Kerstin
A1  - Roth, Susanne
A1  - Behrends, Christian
A1  - Oswald, Franz
A1  - Solbach, Christine
A1  - Louwen, Frank
A1  - Kreis, Nina-Naomi
A1  - Yuan, Juping
T1  - RITA modulates cell migration and invasion by affecting focal adhesion dynamics
T2  - Molecular Oncology
N2  - RITA, the RBP‐J interacting and tubulin‐associated protein, has been reported to be related to tumor development, but the underlying mechanisms are not understood. Since RITA interacts with tubulin and coats microtubules of the cytoskeleton, we hypothesized that it is involved in cell motility. We show here that depletion of RITA reduces cell migration and invasion of diverse cancer cell lines and mouse embryonic fibroblasts. Cells depleted of RITA display stable focal adhesions (FA) with elevated active integrin, phosphorylated focal adhesion kinase, and paxillin. This is accompanied by enlarged size and disturbed turnover of FA. These cells also demonstrate increased polymerized tubulin. Interestingly, RITA is precipitated with the lipoma‐preferred partner (LPP), which is critical in actin cytoskeleton remodeling and cell migration. Suppression of RITA results in reduced LPP and α‐actinin at FA leading to compromised focal adhesion turnover and actin dynamics. This study identifies RITA as a novel crucial player in cell migration and invasion by affecting the turnover of FA through its interference with the dynamics of actin filaments and microtubules. Its deregulation may contribute to malignant progression.
KW  - FAK
KW  - focal adhesion
KW  - integrin
KW  - invasion
KW  - LPP
KW  - RITA
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57403
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-574033
SN  - 2052-9635
VL  - 13
IS  - 10
SP  - 2121
EP  - 2141
PB  - OA Publishing
CY  - London
ER  -