TY  - JOUR
A1  - Mertinkus, Klara R.
A1  - Grün, J. Tassilo
A1  - Altınçekiç, Nadide
A1  - Bains, Jasleen Kaur
A1  - Ceylan, Betül
A1  - Ferner, Jan
A1  - Frydman, Lucio
A1  - Fürtig, Boris
A1  - Hengesbach, Martin
A1  - Hohmann, Katharina Felicitas
A1  - Hymon, Daniel
A1  - Kim, Jihyun
A1  - Knežić, Božana
A1  - Novakovic, Mihajlo
A1  - Oxenfarth, Andreas
A1  - Peter, Stephen
A1  - Qureshi, Nusrat
A1  - Richter, Christian
A1  - Scherf, Tali
A1  - Schlundt, Andreas
A1  - Schnieders, Robbin
A1  - Schwalbe, Harald
A1  - Stirnal, Elke
A1  - Sudakov, Alexey
A1  - Vögele, Jennifer
A1  - Wacker, Anna
A1  - Weigand, Julia
A1  - Wirmer-Bartoschek, Julia
A1  - Wirtz Martin, Maria A.
A1  - Wöhnert, Jens
T1  - 1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2
T2  - Biomolecular NMR assignments
N2  - The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5′- and 3′-genomic ends that are highly conserved among Betacoronaviruses. These genomic ends contain structured cis-acting RNA elements, which are involved in the regulation of viral replication and translation. Structural information about these potential antiviral drug targets supports the development of novel classes of therapeutics against COVID-19. The highly conserved branched stem-loop 5 (SL5) found within the 5′-untranslated region (5′-UTR) consists of a basal stem and three stem-loops, namely SL5a, SL5b and SL5c. Both, SL5a and SL5b feature a 5′-UUUCGU-3′ hexaloop that is also found among Alphacoronaviruses. Here, we report the extensive 1H, 13C and 15N resonance assignment of the 37 nucleotides (nts) long sequence spanning SL5b and SL5c (SL5b + c), as basis for further in-depth structural studies by solution NMR spectroscopy.
KW  - SARS-CoV-2
KW  - 5′-UTR
KW  - SL5b + c
KW  - SL5b
KW  - SL5c
KW  - Solution NMR spectroscopy
KW  - COVID19-NMR
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69570
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-695704
SN  - 1874-270X
N1  - Work at BMRZ is supported by the state of Hesse. Work in COVID19-NMR was supported by the Goethe Corona Funds, by the IWB-EFRE-programme 20007375 of state of Hesse, and the DFG in CRC902: “Molecular Principles of RNA-based regulation.” and infrastructure funds (Project Numbers: 277478796, 277479031, 392682309, 452632086, 70653611). Work at Weizmann was supported by the EU Horizon 2020 program (FET-OPEN Grant 828946, PATHOS), Israel Science Foundation Grants 965/18 and 3572/20, and the Perlman Family Foundation.
N1  - Open Access funding enabled and organized by Projekt DEAL.
N1  - Data availability
We updated the BMRB deposition for SL5b + c with code 50339. Data of SL5b_GC are found in BMRB code 51138 and SL5c BRMB code 51137.
VL  - 16
IS  - 1
SP  - 17
EP  - 25
PB  - Springer Netherlands
CY  - Dordrecht [u.a.]
ER  -