TY  - JOUR
A1  - Hinz, Andreas
A1  - Jedamzick, Johanna
A1  - Herbring, Valentina
A1  - Fischbach, Hanna
A1  - Hartmann, Jessica
A1  - Parcej, David
A1  - Koch, Joachim
A1  - Tampé, Robert
T1  - Assembly and function of the major histocompatibility complex (MHC) I peptide-loading complex are conserved across higher vertebrates
T2  - Journal of biological chemistry
N2  - Antigen presentation to cytotoxic T lymphocytes via major histocompatibility complex class I (MHC I) molecules depends on the heterodimeric transporter associated with antigen processing (TAP). For efficient antigen supply to MHC I molecules in the ER, TAP assembles a macromolecular peptide-loading complex (PLC) by recruiting tapasin. In evolution, TAP appeared together with effector cells of adaptive immunity at the transition from jawless to jawed vertebrates and diversified further within the jawed vertebrates. Here, we compared TAP function and interaction with tapasin of a range of species within two classes of jawed vertebrates. We found that avian and mammalian TAP1 and TAP2 form heterodimeric complexes across taxa. Moreover, the extra N-terminal domain TMD0 of mammalian TAP1 and TAP2 as well as avian TAP2 recruits tapasin. Strikingly, however, only TAP1 and TAP2 from the same taxon can form a functional heterodimeric translocation complex. These data demonstrate that the dimerization interface between TAP1 and TAP2 and the tapasin docking sites for PLC assembly are conserved in evolution, whereas elements of antigen translocation diverged later in evolution and are thus taxon specific.
KW  - ABC Transporter
KW  - Antigen Processing
KW  - Immunology
KW  - Major Histocompatibility Complex (MHC)
KW  - Membrane Protein
KW  - Transporter
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76989
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-769897
SN  - 0021-9258
VL  - 289.2014
IS  - 48
SP  - 33109
EP  - 33117
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -