TY  - JOUR
A1  - Fröb, Luisa
A1  - Rüttermann, Stefan
A1  - Romanos, Georgios
A1  - Herrmann, Eva
A1  - Gerhardt-Szép, Susanne
T1  - Cytotoxicity of self-etch versus etch-and-rinse dentin adhesives: A screening study
T2  - Materials
N2  - Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved.
KW  - cells
KW  - cytotoxicity
KW  - dentin adhesives
KW  - fibroblasts
KW  - in vitro
KW  - screening
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51842
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-518425
SN  - 1996-1944
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 13
IS  - 2, Art. 452
SP  - 1
EP  - 17
PB  - MDPI
CY  - Basel
ER  -