TY  - JOUR
A1  - Kramer, Isabelle Marie
A1  - Baral, Sunita
A1  - Gautam, Ishan
A1  - Braun, Markus
A1  - Magdeburg, Axel
A1  - Phuyal, Parbati
A1  - Kadel, Indira
A1  - Dhimal, Meghnath
A1  - Ahrens, Bodo
A1  - Groneberg, Jan David Alexander
A1  - Müller, Ruth
T1  - STtech: sampling and transport techniques for Aedes eggs during a sampling campaign in a low-resource setting
T2  - Entomologia experimentalis et applicata
N2  - Container-breeding Aedes spp. (Diptera: Culicidae) mosquitoes can be surveilled at low cost using ovitraps. Hence, this method is a preferred monitoring approach of dengue vectors in low-resource settings. The ovitraps consist of a cup filled with water and an oviposition substrate for female mosquitoes. The attractiveness of the substrates for female mosquitoes can greatly differ due to differences in texture, color, and smell of the materials used. We compare four oviposition substrates, which are all low priced, easy to transport, and easy to purchase, to maximize the success of Aedes egg sampling. Sampled egg material is often reared to adulthood for further taxonomic identification and transported to (international) laboratories for specialized vector research. Here we introduce a transport technique for sampled eggs. In addition, we explored the impact of international transport by means of a bilateral hatching experiment in Nepal, the country of origin, and in Germany, in a laboratory specialized in ecophysiological research. The best low-cost oviposition substrate for the dengue vectors Aedes albopictus (Skuse) and Aedes aegypti (L.) was found to be a white cotton sheet. The introduced transport technique of sampled eggs is easy to build from laboratory and household materials and ensures good transport conditions (i.e., temperature and relative humidity). Even under good temperature (17.4–31.0 °C) and humidity conditions (58.9–94.2%), hatching success of eggs was found to be reduced after international transport to Germany when compared to the hatching success of eggs in Nepal. We postulate that air pressure during international transport may have reduced the hatching success and strongly recommend pressure-regulated transport boxes for egg transport via airplane. As the proposed operation procedure is useful in assisting the monitoring of Ae. albopictus and Ae. aegypti in low-resource settings, Aedes researchers are encouraged to follow it for the sampling and transport of Aedes eggs.
KW  - Aedes albopictus
KW  - Aedes aegypti
KW  - oviposition substrate
KW  - egg sampling
KW  - hatching
KW  - international transport
KW  - monitoring
KW  - oviposition
KW  - ovitrap
KW  - Diptera
KW  - Culicidae
KW  - dengue vector
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62163
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-621634
SN  - 1570-7458
N1  - Bundesministerium für Bildung und Forschung. Grant Number: 01Kl1717
VL  - 169
IS  - 4
SP  - 374
EP  - 383
PB  - Wiley-Blackwell
CY  - Oxford [u.a.]
ER  -