TY  - INPR
A1  - Klevanski, Maja
A1  - Herrmannsdörfer, Frank
A1  - Venkataramani, Varun
A1  - Saß, Steffen
A1  - Heilemann, Mike
A1  - Kuner, Thomas
T1  - Automated highly multiplexed super-resolution imaging of protein nano-architecture in cells and tissues
T2  - bioRxiv
N2  - Understanding the nano-architecture of protein machines in diverse sub-cellular compartments remains a challenge despite rapid progress in super-resolution microscopy. While singlemolecule localization microscopy techniques allow the visualization and identification of cellular structures with near-molecular resolution, multiplex-labeling of tens of target proteins within the same sample has not yet been achieved routinely. However, single sample multiplexing is essential to detect patterns that threaten to get lost in multi-sample averaging. Here, we report maS3TORM (multiplexed automated serial staining stochastic optical reconstruction microscopy), a microscopy approach capable of fully automated 3D dSTORM imaging and solution exchange employing a re-staining protocol to achieve highly multiplexed protein localization within individual biological samples. We demonstrate 3D super-resolution images of 15 target proteins in single cultured cells and 16 targets in individual neuronal tissue samples with <10 nm localization precision. This allowed us to define novel nano-architectural features of protein distribution within the presynaptic nerve terminal.
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72613
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-726134
IS  - 791053
ER  -