TY  - JOUR
A1  - Hussain, Shobbir
A1  - Benavente, Sandra Blanco
A1  - Nascimento, Elisabete
A1  - Dragoni, Ilaria
A1  - Kurowski, Agata
A1  - Gillich, Astrid
A1  - Humphreys, Peter
A1  - Frye, Michaela
T1  - The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability
T2  - The journal of cell biology
N2  - Myc-induced SUN domain–containing protein (Misu or NSun2) is a nucleolar RNA methyltransferase important for c-Myc–induced proliferation in skin, but the mechanisms by which Misu contributes to cell cycle progression are unknown. In this study, we demonstrate that Misu translocates from the nucleoli in interphase to the spindle in mitosis as an RNA–protein complex that includes 18S ribosomal RNA. Functionally, depletion of Misu caused multiple mitotic defects, including formation of unstructured spindles, multipolar spindles, and chromosome missegregation, leading to aneuploidy and cell death. The presence of both RNA and Misu is required for correct spindle assembly, and this process is independent of active translation. Misu might mediate its function at the spindle by recruiting nucleolar and spindle-associated protein (NuSAP), an essential microtubule-stabilizing and bundling protein. We further identify NuSAP as a novel direct target gene of c-Myc. Collectively, our results suggest a novel mechanism by which c-Myc promotes proliferation by stabilizing the mitotic spindle in fast-dividing cells via Misu and NuSAP.
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/7370
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-70098
N1  - © 2009 Hussain et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication
date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a
Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license,
as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
VL  - 186
IS  - 1
SP  - 27
EP  - 40
ER  -