TY  - JOUR
A1  - Metzler, Melinda
A1  - Tharyan, Rebecca George
A1  - Klann, Kevin
A1  - Grikscheit, Katharina
A1  - Bojkova, Denisa
A1  - Cinatl, Jindrich
A1  - Tascher, Georg
A1  - Ciesek, Sandra
A1  - Münch, Christian
T1  - SARS-CoV-2 variants show different host cell proteome profiles with delayed immune response activation in Omicron-infected cells
T2  - Molecular & cellular proteomics
N2  - The ancestral SARS-CoV-2 strain that initiated the Covid-19 pandemic at the end of 2019 has rapidly mutated into multiple variants of concern with variable pathogenicity and increasing immune escape strategies. However, differences in host cellular antiviral responses upon infection with SARS-CoV-2 variants remain elusive. Leveraging whole-cell proteomics, we determined host signaling pathways that are differentially modulated upon infection with the clinical isolates of the ancestral SARS-CoV-2 B.1 and the variants of concern Delta and Omicron BA.1. Our findings illustrate alterations in the global host proteome landscape upon infection with SARS-CoV-2 variants and the resulting host immune responses. Additionally, viral proteome kinetics reveal declining levels of viral protein expression during Omicron BA.1 infection when compared to ancestral B.1 and Delta variants, consistent with its reduced replication rates. Moreover, molecular assays reveal deferral activation of specific host antiviral signaling upon Omicron BA.1 and BA.2 infections. Our study provides an overview of host proteome profile of multiple SARS-CoV-2 variants and brings forth a better understanding of the instigation of key immune signaling pathways causative for the differential pathogenicity of SARS-CoV-2 variants.
KW  - Proteomics
KW  - Covid-19
KW  - SARS-CoV-2
KW  - Omicron
KW  - Interferon
KW  - NF-kB
KW  - IRF-3
KW  - Protein-translocation
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/78934
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-789346
SN  - 1535-9476
VL  - 22
IS  - 5, art. 100537
PB  - Elsevier
CY  - Amsterdam
ER  -