TY  - JOUR
A1  - Hubloher, Josephine Joy
A1  - Zeidler, Sabine
A1  - Lamosa, Pedro
A1  - Santos, Helena
A1  - Averhoff, Beate
A1  - Müller, Volker
T1  - Trehalose‐6‐phosphate‐mediated phenotypic change in Acinetobacter baumannii
T2  - Environmental microbiology
N2  - The stress protectant trehalose is synthesized in Acinetobacter baumannii from UPD‐glucose and glucose‐6‐phosphase via the OtsA/OtsB pathway. Previous studies proved that deletion of otsB led to a decreased virulence, the inability to grow at 45°C and a slight reduction of growth at high salinities indicating that trehalose is the cause of these phenotypes. We have questioned this conclusion by producing ∆otsA and ∆otsBA mutants and studying their phenotypes. Only deletion of otsB, but not deletion of otsA or otsBA, led to growth impairments at high salt and high temperature. The intracellular concentrations of trehalose and trehalose‐6‐phosphate were measured by NMR or enzymatic assay. Interestingly, none of the mutants accumulated trehalose any more but the ∆otsB mutant with its defect in trehalose‐6‐phosphate phosphatase activity accumulated trehalose‐6‐phosphate. Moreover, expression of otsA in a ∆otsB background under conditions where trehalose synthesis is not induced led to growth inhibition and the accumulation of trehalose‐6‐phosphate. Our results demonstrate that trehalose‐6‐phosphate affects multiple physiological activities in A. baumannii ATCC 19606.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56555
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-565552
SN  - 1462-2920
SN  - 1462-2912
VL  - 2002
PB  - Blackwell
CY  - Oxford [u.a.]
ER  -