TY  - JOUR
A1  - Langheinrich, Melanie
A1  - Wirtz, Stefan
A1  - Kneis, Barbara
A1  - Gittler, Matthias M.
A1  - Tyc, Olaf
A1  - Schierwagen, Robert
A1  - Brunner, Maximilian
A1  - Krautz, Christian
A1  - Weber, Georg F.
A1  - Pilarsky, Christian
A1  - Trebicka, Jonel
A1  - Agaimy, Abbas
A1  - Grützmann, Robert
A1  - Kersting, Stephan
T1  - Microbiome patterns in matched bile, duodenal, pancreatic tumor tissue, drainage, and stool samples: association with preoperative stenting and postoperative pancreatic fistula development
T2  - Journal of Clinical Medicine
N2  - Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.
KW  - microbiome
KW  - pancreatic surgery
KW  - postoperative complications
KW  - pancreatic cancer
KW  - 16S rRNA sequencing
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56052
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-560524
SN  - 2077-0383
VL  - 9
IS  - (9), 2785
PB  - MDPI
CY  - Basel
ER  -