TY  - JOUR
A1  - Nasirzade, Jila
A1  - Kargarpour, Zahra
A1  - Panahipour, Layla
A1  - Schwarz, Frank
A1  - Gruber, Reinhard
T1  - Cleaning teeth reduces the inflammatory response of macrophages to acid dentine lysate
T2  - International journal of molecular sciences
N2  - Particulate autogenous tooth roots are used for alveolar bone augmentation surgery; however, dental plaque may provoke an inflammatory response that may counteract the desired graft consolidation process. Traditional mechanical cleaning of extracted teeth may be of support to lower a possible inflammatory response of the autograft. To test this assumption, extracted porcine teeth were left either uncleaned or underwent mechanical cleaning with a toothbrush and toothpaste before being fragmented and subjected to acid lysis, termed as unclean acid dentine lysate (ucADL) and clean acid dentine lysate (cADL), respectively. The inflammatory responses of murine macrophage RAW 264.7 cells being exposed to the respective acid dentine lysates were evaluated at the level of inflammatory gene expression and IL6 immunoassays. We report here that acid lysates obtained from uncleaned teeth provoked a robust increase in IL1β, IL6, and COX2 in RAW 264.7 cells. The mechanical removal of dental plaque significantly reduced the inflammatory response. Consistently, Limulus tests revealed that tooth cleaning lowers the presence of endotoxins in dentine lysates. To further prove the involvement of endotoxins, a toll-like receptor 4 (TLR4) inhibitor TAK242 was introduced. TAK242 abolished the inflammatory response provoked by acid lysates obtained from uncleaned teeth in RAW 264.7 cells. Moreover, nuclear translocation and phosphorylation of the TLR4 downstream NFκB-p65 were attenuated at the presence of cleaned versus uncleaned dentine lysates. Taken together, our data support the importance of dental plaque removal of teeth being extracted for alveolar bone augmentation surgery.
KW  - autograft
KW  - tooth
KW  - transplantation
KW  - augmentation
KW  - dentistry
KW  - inflammation
KW  - macrophages
KW  - LPS
KW  - acid dentine lysate
KW  - cleaning
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56949
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-569499
SN  - 1422-0067
VL  - 21
IS  - 9207
PB  - MDPI
CY  - Basel
ER  -