TY  - JOUR
A1  - Herrera-Vizcaíno, Carlos
A1  - Dohle, Eva
A1  - Maawi, Sarah al-
A1  - Booms, Patrick
A1  - Sader, Robert Alexander
A1  - Kirkpatrick, Charles James
A1  - Choukroun, Joseph
A1  - Ghanaati, Shahram Michael
T1  - Platelet-rich fibrin secretome induces three dimensional angiogenic activation in vitro
T2  - European cells & materials
N2  - Different tissue engineering techniques are used to support rapid vascularisation. A novel technique is the use of platelet-rich fibrin (PRF), an autologous source of growth factors. This study was the first to investigate the influence of PRF matrices, isolated following different centrifugation protocols, on human dermal vascular endothelial cells (ECs) in mono-culture and co-culture with human primary fibroblasts (HFs) as an in vitro model for tissue regeneration. Focus was placed on vascular structure formation and growth factor release. HFs and ECs were cultivated with PRF prepared using a high (710 ×g) or low (44 ×g) relative centrifugation force (RCF) over 14 d. Immunofluorescence staining and immunohistochemistry were used to evaluate the microvascular formation. Cell culture supernatants were collected for evaluation of growth factor release. The results showed a PRF-mediated effect on the induction of angiogenesis in ECs. Microvessel-like structure formation was promoted when ECs were combined with low-RCF PRF as compared to high-RCF PRF or control group. The percentage of vascular lumen area was significantly higher in low-RCF PRF, especially at day 7, which coincided with statistically significantly higher growth factor [vascular endothelial factor (VEGF), transforming growth factor β1 (TGF-β1) and platelet derived growth factor (PDGF)] concentration measured in low-RCF PRF as compared to high-RCF PRF or control group. In conclusion, reducing the RCF according to the low-speed centrifugation concept (LSCC) resulted in increased growth factor release and angiogenic structure formation with EC mono-culture, suggesting that PRF may be a highly beneficial therapeutic tool for tissue engineering applications.
KW  - Platelet-rich fibrin
KW  - low speed centrifugation concept
KW  - angiogenesis
KW  - secretome
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50075
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-500757
SN  - 1473-2262
N1  - Copyright policy: This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by-sa/4.0/).
VL  - 37
SP  - 250
EP  - 264
PB  - Univ. of Wales
CY  - Aberystwyth, Wales
ER  -