TY  - JOUR
A1  - Hinzke, Tjorven
A1  - Kleiner, Manuel
A1  - Meister, Mareike
A1  - Schlüter, Rabea
A1  - Hentschker, Christian
A1  - Pané-Farré, Jan
A1  - Hildebrandt, Petra
A1  - Felbeck, Horst
A1  - Sievert, Stefan M.
A1  - Bonn, Florian
A1  - Völker, Uwe
A1  - Becher, Dörte
A1  - Schweder, Thomas
A1  - Markert, Stephanie
T1  - Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis
T2  - eLife
N2  - The hydrothermal vent tubeworm Riftia pachyptila hosts a single 16S rRNA phylotype of intracellular sulfur-oxidizing symbionts, which vary considerably in cell morphology and exhibit a remarkable degree of physiological diversity and redundancy, even in the same host. To elucidate whether multiple metabolic routes are employed in the same cells or rather in distinct symbiont subpopulations, we enriched symbionts according to cell size by density gradient centrifugation. Metaproteomic analysis, microscopy, and flow cytometry strongly suggest that Riftia symbiont cells of different sizes represent metabolically dissimilar stages of a physiological differentiation process: While small symbionts actively divide and may establish cellular symbiont-host interaction, large symbionts apparently do not divide, but still replicate DNA, leading to DNA endoreduplication. Moreover, in large symbionts, carbon fixation and biomass production seem to be metabolic priorities. We propose that this division of labor between smaller and larger symbionts benefits the productivity of the symbiosis as a whole.
KW  - Riftia pachyptila
KW  - cell differentiation
KW  - cell heterogeneity
KW  - host-microbe interaction
KW  - Microbiology and Infectious Disease
KW  - sulfur-oxidizing symbiont
KW  - symbiosis
KW  - Research Article
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/83786
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-837866
SN  - 2050-084X
N1  - The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (ProteomeXchange - ProteomeCentral) via the PRIDE partner repository (Vizcaíno et al., 2016) with the dataset identifier PXD016986.
N1  - Funding: Open Access Publication Fund of the University of Greifswald
N1  - Funding: DFG ; 393148499
N1  - Funding: National Institute of Food and Agriculture ; Hatch project 1014212
N1  - Funding: National Science Foundation ; OCE-1131095
N1  - Funding: German Research Foundation ; MA6346/2–1
N1  - Funding: National Science Foundation ; OCE-1559198
N1  - Funding: Institut für Marine Biotechnologie e.V.
N1  - Funding: German Academic Exchange Service
N1  - Funding: NC State University (Chancellor's Faculty Excellence Program Cluster on Microbiomes and Complex Microbial Communities)
N1  - Funding: Woods Hole Oceanographic Institution (The WHOI Investment in Science Fund)
VL  - 10
IS  - art. e58371
SP  - 1
EP  - 45
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -