TY  - INPR
A1  - Xing, Huaipeng
A1  - Taniguchi, Reiya
A1  - Khusainov, Iskander
A1  - Kreysing, Jan Philipp
A1  - Welsch, Sonja
A1  - Turoňová, Beata
A1  - Beck, Martin
T1  - Translation dynamics in human cells visualized at high-resolution reveal cancer drug action
T2  - bioRxiv
N2  - Ribosomes catalyze protein synthesis by cycling through various functional states. These states have been extensively characterized in vitro, yet their distribution in actively translating human cells remains elusive. Here, we optimized a cryo-electron tomography-based approach and resolved ribosome structures inside human cells with a local resolution of up to 2.5 angstroms. These structures revealed the distribution of functional states of the elongation cycle, a Z tRNA binding site and the dynamics of ribosome expansion segments. In addition, we visualized structures of Homoharringtonine, a drug for chronic myeloid leukemia treatment, within the active site of the ribosome and found that its binding reshaped the landscape of translation. Overall, our work demonstrates that structural dynamics and drug effects can be assessed at near-atomic detail within human cells.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72380
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-723801
IS  - 2023.03.02.529652
ER  -