TY  - JOUR
A1  - Spille, Jan-Hendrik
A1  - Kaminski, Tim P.
A1  - Scherer, Katharina
A1  - Rinne, Jennifer S.
A1  - Heckel, Alexander
A1  - Kubitscheck, Ulrich
T1  - Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking
T2  - Nucleic acids research
N2  - Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light microscopic particle observation is usually limited to two-dimensional projections of short trajectory segments, we report here image-based real-time three-dimensional single particle tracking in an active feedback loop with single molecule sensitivity. We tracked particles carrying only 1-3 fluorophores deep inside living tissue with high spatio-temporal resolution. Using this approach, we succeeded to acquire trajectories containing several hundred localizations. We present statistical methods to find significant deviations from random Brownian motion in such trajectories. The analysis allowed us to directly observe transitions in the mobility of ribosomal (r)RNA and Balbiani ring (BR) messenger (m)RNA particles in living Chironomus tentans salivary gland cell nuclei. We found that BR mRNA particles displayed phases of reduced mobility, while rRNA particles showed distinct binding events in and near nucleoli.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/35830
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-358307
UR  - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333372/
SN  - 1362-4962
SN  - 0305-1048
N1  - © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 43
IS  - (2): e14
SP  - 1
EP  - 11
PB  - Oxford Univ. Press
CY  - Oxford
ER  -