TY  - JOUR
A1  - Pittner, Stefan
A1  - Merold, Veronika
A1  - Anders, Sven
A1  - Lohner, Larissa
A1  - Amendt, Jens
A1  - Klinger, Miriam
A1  - Hausmann, Roland
A1  - Kißling, Steffen
A1  - Monticelli, Fabio Carlo
A1  - Geissenberger, Janine
A1  - Zissler, Angela
A1  - Steinbacher, Peter
T1  - A standard protocol for the analysis of postmortem muscle protein degradation: process optimization and considerations for the application in forensic PMI estimation
T2  - International journal of legal medicine
N2  - The analysis of postmortem protein degradation has become of large interest for the estimation of the postmortem interval (PMI). Although several techniques have been published in recent years, protein degradation-based techniques still largely did not exceed basic research stages. Reasons include impractical and complex sampling procedures, as well as highly variable protocols in the literature, making it difficult to compare results. Following a three-step procedure, this study aimed to establish an easily replicable standardized procedure for sampling and processing, and further investigated the reliability and limitations for routine application. Initially, sampling and processing were optimized using a rat animal model. In a second step, the possible influences of sample handling and storage on postmortem protein degradation dynamics were assessed on a specifically developed human extracorporeal degradation model. Finally, the practical application was simulated by the collection of tissue in three European forensic institutes and an international transfer to our forensic laboratory, where the samples were processed and analyzed according to the established protocol.
KW  - Protocol
KW  - Muscle
KW  - Protein
KW  - Degradation
KW  - PMI
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69460
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-694603
SN  - 1437-1596
N1  - Open access funding provided by Austrian Science Fund (FWF). This work was supported by the Austrian Science Fund (FWF), grant P31490.
VL  - 136
SP  - 1913
EP  - 1923
PB  - Springer
CY  - Berlin ; Heidelberg
ER  -