TY  - JOUR
A1  - Latreille, Phil
A1  - Norton, Stacie
A1  - Goldman, Barry S.
A1  - Henkhaus, John
A1  - Miller, Nancy
A1  - Barbazuk, Brad
A1  - Bode, Helge Björn
A1  - Darby, Creg
A1  - Du, Zijin
A1  - Forst, Steve
A1  - Gaudriault, Sophie
A1  - Goodner, Brad
A1  - Goodrich-Blair, Heidi
A1  - Slater, Steven
T1  - Optical mapping as a routine tool for bacterial genome sequence finishing
T2  - BMC genomics
N2  - Background: In sequencing the genomes of two Xenorhabdus species, we encountered a large number of sequence repeats and assembly anomalies that stalled finishing efforts. This included a stretch of about 12 Kb that is over 99.9% identical between the plasmid and chromosome of X. nematophila.

Results: Whole genome restriction maps of the sequenced strains were produced through optical mapping technology. These maps allowed rapid resolution of sequence assembly problems, permitted closing of the genome, and allowed correction of a large inversion in a genome assembly that we had considered finished.

Conclusion: Our experience suggests that routine use of optical mapping in bacterial genome sequence finishing is warranted. When combined with data produced through 454 sequencing, an optical map can rapidly and inexpensively generate an ordered and oriented set of contigs to produce a nearly complete genome sequence assembly.
Y1  - 2007
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/27141
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-271412
SN  - 1471-2164
N1  - © 2007 Latreille et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 8
IS  - 321
PB  - BioMed Central
CY  - London
ER  -