TY  - JOUR
A1  - Trojan, Jörg
A1  - Klein-Scory, Susanne
A1  - Koch, Christine
A1  - Schmiegel, Wolff
A1  - Baraniskin, Alexander
T1  - Clinical application of liquid biopsy in targeted therapy of metastatic colorectal cancer
T2  - Case Reports in Oncological Medicine
N2  - Background. Colorectal cancers (CRC) shed DNA into blood circulation. There is growing evidence that the analysis of circulating tumor DNA can be effectively used for monitoring of disease, to track tumor heterogeneity and to evaluate response to treatment. Case Presentation. Here, we describe two cases of patients with advanced CRC. The first case is about a patient with no available tissue for analysis of RAS mutation status. Liquid biopsy revealed RAS-wild-type and the therapy with anti-EGFR (epidermal growth factor receptor) monoclonal antibody cetuximab could be initiated. In the second case, the mutational profile of a patient with initial wild-type RAS-status was continually tracked during the course of treatment. An acquired KRAS exon 3 mutation was detected. The number of KRAS mutated fragments decreased continuously after the discontinuation of the therapy with EGFR-specific antibodies. Conclusion. Liquid biopsy provides a rapid genotype result, which accurately reproduces the current mutation status of tumor tissue. Furthermore, liquid biopsy enables close monitoring of the onset of secondary resistance to anti-EGFR therapy.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/42920
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-429204
N1  - Copyright © 2017 Jörg Trojan et al. This is an open access article distributed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2017
IS  - Article ID 6139634
ER  -