TY - JOUR A1 - Rudolf, Martin A1 - Czajka, Christina A1 - Börstler, Jessica A1 - Melaun, Christian A1 - Jöst, Hanna A1 - Thien, Heidrun von A1 - Badusche, Marlis A1 - Becker, Norbert A1 - Schmidt-Chanasit, Jonas A1 - Krüger, Andreas A1 - Tannich, Egbert A1 - Becker, Stefanie T1 - First nationwide surveillance of Culex pipiens complex and Culex torrentium mosquitoes demonstrated the presence of Culex pipiens biotype pipiens/molestus hybrids in Germany T2 - PLoS One N2 - Mosquitoes and other arthropods may transmit medically important pathogens, in particular viruses such as West Nile virus. The presence of suitable hosts and competent vectors for those zoonotic viruses is essential for an enzootic transmission, which is a prerequisite for epidemics. To establish reliable risk projections, it is an urgent need for an exact identification of mosquito species, which is especially challenging in the case of sibling species, such as Culex. pipiens pipiens biotypes pipiens and molestus. To facilitate detection of different Culex pipiens forms and their hybrids we established a multiplex real-time PCR. Culex pipiens samples were obtained by egg raft collection and rearing until imago stage or adult sampling using CO2 baited traps and gravid traps. In total, we tested more than 16,500 samples collected all over Germany in the years 2011 and 2012. The predominant species in Germany are Culex pipiens pipiens biotype pipiens and Culex. torrentium, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes at sites where both species occur sympatrically. This report of a potentially important bridge vector for West Nile virus might have major impact in the risk projections for West Nile virus in Germany. Y1 - 2013 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/31626 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-316269 SN - 1932-6203 N1 - Copyright: © 2013 Rudolf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. VL - 8 IS - (9):e71832 PB - PLoS CY - Lawrence, Kan. ER -