TY  - INPR
A1  - Acera Mateos, Pablo
A1  - Sethi, Aditya J.
A1  - Guarnacci, Marco
A1  - Ravindran, Agin
A1  - Srivastava, Akanksha
A1  - Xu, Jiajia
A1  - Woodward, Katrina
A1  - Hamilton, William
A1  - Gao, Jing
A1  - Starrs, Lora M.
A1  - Burgio, Gaetan
A1  - Hayashi, Rippei
A1  - Wickramasinghe, Vihandha
A1  - Dehorter, Nathalie
A1  - Preiss, Thomas
A1  - Shirokikh, Nikolay
A1  - Eyras Jiménez, Eduardo Angel
T1  - Identification of m6A and m5C RNA modifications at single-molecule resolution from Nanopore sequencing
T2  - bioRxiv
N2  - The expanding field of epitranscriptomics might rival the epigenome in the diversity of the biological processes impacted. However, the identification of modifications in individual RNA molecules remains challenging. We present CHEUI, a new method that detects N6-methyladenosine (m6A) and 5-methylcytidine (m5C) at single-nucleotide and single-molecule resolution from Nanopore signals. CHEUI predicts methylation in Nanopore reads and transcriptomic sites in a single condition, and differential m6A and m5C methylation between any two conditions. Using extensive benchmarking with Nanopore data derived from synthetic and natural RNA, CHEUI showed higher accuracy than other existing methods in detecting m6A and m5C sites and quantifying the site stoichiometry levels, while maintaining a lower proportion of false positives. CHEUI provides a new capability to detect RNA modifications with high accuracy and resolution that can be cost-effectively expanded to other modifications to unveil the full span of the epitranscriptome in normal and disease conditions.
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/84495
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-844955
UR  - https://www.biorxiv.org/content/10.1101/2022.03.14.484124v2
IS  - 2022.03.14.484124 Version 2
PB  - bioRxiv
ER  -