TY  - JOUR
A1  - Sönmez, Tolga Taha
A1  - Al-Sawaf, Othman
A1  - Brandacher, Gerald
A1  - Kanzler, Isabella
A1  - Tuchscheerer, Nancy
A1  - Tohidnezhad, Mersedeh
A1  - Kanatas, Anastasios
A1  - Knobe, Matthias
A1  - Fragoulis, Athanassios
A1  - Tolba, René H.
A1  - Mitchell, David
A1  - Pufe, Thomas
A1  - Wruck, Christoph Jan
A1  - Hölzle, Frank
A1  - Liehn, Elisa Anamaria
T1  - A novel laser-doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research
T2  - PLoS One
N2  - Suitable and reproducible experimental models of translational research in reconstructive surgery that allow in-vivo investigation of diverse molecular and cellular mechanisms are still limited. To this end we created a novel murine model of acute hindlimb ischemia-reperfusion to mimic a microsurgical free flap procedure. Thirty-six C57BL6 mice (n = 6/group) were assigned to one control and five experimental groups (subject to 6, 12, 96, 120 hours and 14 days of reperfusion, respectively) following 4 hours of complete hindlimb ischemia. Ischemia and reperfusion were monitored using Laser-Doppler Flowmetry. Hindlimb tissue components (skin and muscle) were investigated using histopathology, quantitative immunohistochemistry and immunofluorescence. Despite massive initial tissue damage induced by ischemia-reperfusion injury, the structure of the skin component was restored after 96 hours. During the same time, muscle cells were replaced by young myotubes. In addition, initial neuromuscular dysfunction, edema and swelling resolved by day 4. After two weeks, no functional or neuromuscular deficits were detectable. Furthermore, upregulation of VEGF and tissue infiltration with CD34-positive stem cells led to new capillary formation, which peaked with significantly higher values after two weeks. These data indicate that our model is suitable to investigate cellular and molecular tissue alterations from ischemia-reperfusion such as occur during free flap procedures.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/30090
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-300907
SN  - 1932-6203
N1  - Copyright: © 2013 Sönmez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 8
IS  - (6):e66498
PB  - PLoS
CY  - Lawrence, Kan.
ER  -