TY  - JOUR
A1  - Jesus, Vanessa de
A1  - Biedenbänder, Thomas
A1  - Vögele, Jennifer
A1  - Wöhnert, Jens
A1  - Fürtig, Boris
T1  - NMR assignment of non-modified tRNAIle from Escherichia coli
T2  - Biomolecular NMR assignments
N2  - tRNAs are L-shaped RNA molecules of ~ 80 nucleotides that are responsible for decoding the mRNA and for the incorporation of the correct amino acid into the growing peptidyl-chain at the ribosome. They occur in all kingdoms of life and both their functions, and their structure are highly conserved. The L-shaped tertiary structure is based on a cloverleaf-like secondary structure that consists of four base paired stems connected by three to four loops. The anticodon base triplet, which is complementary to the sequence of the mRNA, resides in the anticodon loop whereas the amino acid is attached to the sequence CCA at the 3′-terminus of the molecule. tRNAs exhibit very stable secondary and tertiary structures and contain up to 10% modified nucleotides. However, their structure and function can also be maintained in the absence of nucleotide modifications. Here, we present the assignments of nucleobase resonances of the non-modified 77 nt tRNAIle from the gram-negative bacterium Escherichia coli. We obtained assignments for all imino resonances visible in the spectra of the tRNA as well as for additional exchangeable and non-exchangeable protons and for heteronuclei of the nucleobases. Based on these assignments we could determine the chemical shift differences between modified and non-modified tRNAIle as a first step towards the analysis of the effect of nucleotide modifications on tRNA’s structure and dynamics.
KW  - tRNA
KW  - RNA
KW  - NMR
KW  - Assignment
KW  - Modification
KW  - Dynamics
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69584
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-695849
SN  - 1874-270X
N1  - Data availability
Chemical shift data is available at the Biological Magnetic Resonance Bank (http://www.bmrb.wisc.edu).
N1  - Open Access funding enabled and organized by Projekt DEAL. This work was funded by the German funding agency (DFG) in Collaborative Research Center 902: Molecular principles of RNA-based regulation.
N1  - Work at Center for Biomolecular Magnetic Resonance (BMRZ) was supported by the state of Hesse (HMWK).
VL  - 16
IS  - 1
SP  - 165
EP  - 170
PB  - Springer Netherlands
CY  - Dordrecht [u.a.]
ER  -