TY  - JOUR
A1  - Troidl, Kerstin
A1  - Schubert, Christian
A1  - Vlacil, Ann-Kathrin
A1  - Chennupati, Ramesh
A1  - Koch, Sören
A1  - Schütt, Jutta
A1  - Oberoi, Raghav
A1  - Schaper, Wolfgang
A1  - Schmitz-Rixen, Thomas
A1  - Schieffer, Bernhard
A1  - Grote, Karsten
T1  - The lipopeptide MALP-2 promotes collateral growth
T2  - Cells
N2  - Beyond their role in pathogen recognition and the initiation of immune defense, Toll-like receptors (TLRs) are known to be involved in various vascular processes in health and disease. We investigated the potential of the lipopeptide and TLR2/6 ligand macrophage activating protein of 2-kDA (MALP-2) to promote blood flow recovery in mice. Hypercholesterolemic apolipoprotein E (Apoe)-deficient mice were subjected to microsurgical ligation of the femoral artery. MALP-2 significantly improved blood flow recovery at early time points (three and seven days), as assessed by repeated laser speckle imaging, and increased the growth of pre-existing collateral arteries in the upper hind limb, along with intimal endothelial cell proliferation in the collateral wall and pericollateral macrophage accumulation. In addition, MALP-2 increased capillary density in the lower hind limb. MALP-2 enhanced endothelial nitric oxide synthase (eNOS) phosphorylation and nitric oxide (NO) release from endothelial cells and improved the experimental vasorelaxation of mesenteric arteries ex vivo. In vitro, MALP-2 led to the up-regulated expression of major endothelial adhesion molecules as well as their leukocyte integrin receptors and consequently enhanced the endothelial adhesion of leukocytes. Using the experimental approach of femoral artery ligation (FAL), we achieved promising results with MALP-2 to promote peripheral blood flow recovery by collateral artery growth.
KW  - TLR2/6
KW  - femoral artery ligation
KW  - blood flow recovery
KW  - collateral growth
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55575
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-555759
SN  - 2073-4409
VL  - 9
IS  - (4), article 997
PB  - MDPI
CY  - Basel
ER  -