TY  - JOUR
A1  - Singh, Sneha
A1  - Akhter, Mohammad Suhail
A1  - Dodt, Johannes
A1  - Volkers, Peter
A1  - Reuter, Andreas
A1  - Reinhart, Christoph
A1  - Krettler, Christoph
A1  - Oldenburg, Johannes
A1  - Biswas, Arijit
T1  - Identification of potential novel interacting partners for coagulation factor xiii b (fxiii-b) subunit, a protein associated with a rare bleeding disorder
T2  - International journal of molecular sciences
N2  - Coagulation factor XIII (FXIII) is a plasma-circulating heterotetrameric pro-transglutaminase complex that is composed of two catalytic FXIII-A and two protective/regulatory FXIII-B subunits. FXIII acts by forming covalent cross-links within a preformed fibrin clots to prevent its premature fibrinolysis. The FXIII-A subunit is known to have pleiotropic roles outside coagulation, but the FXIII-B subunit is a relatively unexplored entity, both structurally as well as functionally. Its discovered roles so far are limited to that of the carrier/regulatory protein of its partner FXIII-A subunit. In the present study, we have explored the co-presence of protein excipients in commercial FXIII plasma concentrate FibrogamminP by combination of protein purification and mass spectrometry-based verification. Complement factor H was one of the co-excipients observed in this analysis. This was followed by performing pull down assays from plasma in order to detect the putative novel interacting partners for the FXIII-B subunit. Complement system proteins, like complement C3 and complement C1q, were amongst the proteins that were pulled down. The only protein that was observed in both experimental set ups was alpha-2-macroglobulin, which might therefore be a putative interacting partner of the FXIII/FXIII-B subunit. Future functional investigations will be needed to understand the physiological significance of this association.
KW  - Factor XIII
KW  - FXIII deficiency
KW  - excipients
KW  - pleiotropy
KW  - mass spectrometry
KW  - complement system
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/53564
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-535640
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 20
IS  - 11, Art. 2682
SP  - 1
EP  - 12
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -