TY  - JOUR
A1  - Krüger, Heike
A1  - Asido, Marvin
A1  - Wachtveitl, Josef
A1  - Tampé, Robert
A1  - Wieneke, Ralph
T1  - Sensitizer-enhanced two-photon patterning of biomolecules in photoinstructive hydrogels
T2  - Communications materials
N2  - Photoresponsive hydrogels can be employed to coordinate the organization of proteins in three dimensions (3D) and thus to spatiotemporally control their physiochemical properties by light. However, reversible and user-defined tethering of proteins and protein complexes to biomaterials pose a considerable challenge as this is a cumbersome process, which, in many cases, does not support the precise localization of biomolecules in the z direction. Here, we report on the 3D patterning of proteins with polyhistidine tags based on in-situ two-photon lithography. By exploiting a two-photon activatable multivalent chelator head, we established the protein mounting of hydrogels with micrometer precision. In the presence of photosensitizers, a substantially enhanced two-photon activation of the developed tool inside hydrogels was detected, enabling the user-defined 3D protein immobilization in hydrogels with high specificity, micrometer-scale precision, and under mild light doses. Our protein-binding strategy allows the patterning of a wide variety of proteins and offers the possibility to dynamically modify the biofunctional properties of materials at defined subvolumes in 3D.
KW  - Biomaterials – proteins
KW  - Chemical tools
KW  - Multiphoton microscopy
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69780
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-697800
SN  - 2662-4443
N1  - This research was supported by the German Research Foundation (GRK 1986 to R.W. and R.T.), the LOEWE program (DynaMem A03 to R.W. and R.T.), the Volkswagen Foundation (Az. 96 498 to R.W. and Az. 96 496 to R.T.) and the Reinhart Koselleck Project (TA 157/12–1 to R.T.).
N1  - Open Access funding enabled and organized by Projekt DEAL.
VL  - 3
IS  - art. 9
SP  - 1
EP  - 12
PB  - Springer Nature
CY  - London
ER  -