TY  - JOUR
A1  - De-Zolt, Silke
A1  - Schnütgen, Frank
A1  - Seisenberger, Claudia
A1  - Hansen, Jens
A1  - Hollatz, Melanie
A1  - Floss, Thomas
A1  - Ruiz, Patricia
A1  - Wurst, Wolfgang
A1  - Melchner, Harald von
T1  - High-throughput trapping of secretory pathway genes in mouse embryonic stem cells
T2  - Nucleic acids research
N2  - High-throughput gene trapping is a random approach for inducing insertional mutations across the mouse genome. This approach uses gene trap vectors that simultaneously inactivate and report the expression of the trapped gene at the insertion site, and provide a DNA tag for the rapid identification of the disrupted gene. Gene trapping has been used by both public and private institutions to produce libraries of embryonic stem (ES) cells harboring mutations in single genes. Presently,~ 66% of the protein coding genes in the mouse genome have been disrupted by gene trap insertions. Among these, however, genes encoding signal peptides or transmembrane domains (secretory genes) are underrepresented because they are not susceptible to conventional trapping methods. Here, we describe a high-throughput gene trapping strategy that effectively targets secretory genes. We used this strategy to assemble a library of ES cells harboring mutations in 716 unique secretory genes, of which 61% were not trapped by conventional trapping, indicating that the two strategies are complementary. The trapped ES cell lines, which can be ordered from the International Gene Trap Consortium (http://www.genetrap.org), are freely available to the scientific community.
Y1  - 2006
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/2790
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-26180
SN  - 1362-4962
SN  - 0305-1048
N1  - © The Author 2006. Published by Oxford University Press. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
VL  - 34
IS  - 3, e25
SP  - 1
EP  - 8
PB  - Oxford Univ. Press
CY  - Oxford
ER  -