TY  - JOUR
A1  - Gerlich, Wolfram H.
A1  - Determann, Helmut
A1  - Wieland, Theodor
T1  - Die Gelchromatographie großer Partikel und ihre Grenzen
T1  - Limitation of particles size in gel chromatography
T2  - Zeitschrift für Naturforschung, B
N2  - The behaviour of molecules and particles of 2 - 400 nm radius in gel chromatography was investigated using sephadex, agarose, and pearl shaped cellulose gel of different porosity. Correlations between elution volumes and particle sizes are given in Fig. 1. We found that particles from ca. 400 nm diameter upwards were more or less irreversibly adsorbed depending on the particle size. So only 15% of rat liver mitochondria and 5% of E. coli bacteria could be eluted from the loose cellulose Cu3. It is assumed that this adsorption is due to the absence of Brownian motion of large particles, which therefore are more or less subject to the gravity and to adsorption forces of the gel.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69856
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-698561
SN  - 1865-7117
SN  - 0932-0776
VL  - 25
IS  - 7
SP  - 1235
EP  - 1239
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -