TY  - JOUR
A1  - Chang, Shu
A1  - Berman, Judit
A1  - Sheng, Yanmin
A1  - Wang, Yingdian
A1  - Capell, Teresa
A1  - Shi, Lianxuan
A1  - Ni, Xiuzhen
A1  - Sandmann, Gerhard
A1  - Christou, Paul
A1  - Zhu, Changfu
T1  - Cloning and functional characterization of the maize (Zea mays L.) carotenoid epsilon hydroxylase gene
T2  - PLoS One
N2  - The assignment of functions to genes in the carotenoid biosynthesis pathway is necessary to understand how the pathway is regulated and to obtain the basic information required for metabolic engineering. Few carotenoid ε-hydroxylases have been functionally characterized in plants although this would provide insight into the hydroxylation steps in the pathway. We therefore isolated mRNA from the endosperm of maize (Zea mays L., inbred line B73) and cloned a full-length cDNA encoding CYP97C19, a putative heme-containing carotenoid ε hydroxylase and member of the cytochrome P450 family. The corresponding CYP97C19 genomic locus on chromosome 1 was found to comprise a single-copy gene with nine introns. We expressed CYP97C19 cDNA under the control of the constitutive CaMV 35S promoter in the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene. The analysis of carotenoid levels and composition showed that lutein accumulated to high levels in the rosette leaves of the transgenic lines but not in the untransformed lut1 mutants. These results allowed the unambiguous functional annotation of maize CYP97C19 as an enzyme with strong zeinoxanthin ε-ring hydroxylation activity.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/37996
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-379969
SN  - 1932-6203
N1  - Copyright: © 2015 Chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 10
IS  - (6): e0128758
SP  - 1
EP  - 14
PB  - PLoS
CY  - Lawrence, Kan.
ER  -