TY  - JOUR
A1  - Gülşen, Şebnem Hazal
A1  - Tileklioglu, Evren
A1  - Bode, Edna
A1  - Cimen, Harun
A1  - Ertabaklar, Hatice
A1  - Uluğ, Derya
A1  - Ertuğ, Sema
A1  - Wenski, Sebastian Leonhard
A1  - Touray, Mustapha
A1  - Hazir, Canan
A1  - Bilecenoglu, Duygu Kaya
A1  - Yildiz, Ibrahim
A1  - Bode, Helge Björn
A1  - Hazir, Selcuk
T1  - Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
T2  - Scientific reports
N2  - Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future.
KW  - Biotechnology
KW  - Drug discovery
KW  - Microbiology
KW  - Molecular biology
KW  - Pathogenesis
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69561
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-695612
SN  - 2045-2322
N1  - Open Access funding enabled and organized by Projekt DEAL. This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK-Project Number: 116S387) and Aydin Adnan Menderes University, Project Number: 20001).
VL  - 12
IS  - art. 10779
SP  - 1
EP  - 13
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - [London]
ER  -