TY  - JOUR
A1  - Strobl, Frederic
A1  - Schetelig, Marc Florian
A1  - Stelzer, Ernst H. K.
T1  - In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development
T2  - Scientific data
N2  - The Mediterranean fruit fly (medfly), Ceratitis capitata, is an important model organism in biology and agricultural research with high economic relevance. However, information about its embryonic development is still sparse. We share nine long-term live imaging datasets acquired with light sheet fluorescence microscopy (484.5 h total recording time, 373 995 images, 256 Gb) with the scientific community. Six datasets show the embryonic development in toto for about 60 hours at 30 minutes intervals along four directions in three spatial dimensions, covering approximately 97% of the entire embryonic development period. Three datasets focus on germ cell formation and head involution. All imaged embryos hatched morphologically intact. Based on these data, we suggest a two-level staging system that functions as a morphogenetic framework for upcoming studies on medfly. Our data supports research on wild-type or aberrant morphogenesis, quantitative analyses, comparative approaches to insect development as well as studies related to pest control. Further, they can be used to test advanced image processing approaches or to train machine learning algorithms and/or neuronal networks.
KW  - Embryogenesis
KW  - Fluorescence imaging
KW  - Light-sheet microscopy
KW  - Morphogenesis
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63273
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-632737
SN  - 2052-4463
N1  - Open Access funding enabled and organized by Projekt DEAL.
N1  - Code availability
The custom Mathematica (Wolfram Research, version 13.0) script used to subject the t stacks to a mean transformation has been described previously (this function does not require any parameters).
VL  - 9
IS  - art. 340
SP  - 1
EP  - 9
PB  - Nature Publ. Group
CY  - London
ER  -