TY  - JOUR
A1  - Hammer, Andreas
A1  - Cerretti, Geraldine
A1  - Ricciardi, Dario Angelo
A1  - Schiffmann, David
A1  - Maranda, Simon
A1  - Kummer, Raphael
A1  - Zumbühl, Christoph
A1  - Rattenbacher-Kiser, Karin F.
A1  - Arx, Silvan von
A1  - Ammann, Sebastian
A1  - Strobl, Frederic
A1  - Berkane, Rayene
A1  - Stolz, Alexandra
A1  - Stelzer, Ernst H. K.
A1  - Egli, Marcel
A1  - Schleiff, Enrico
A1  - Wuest, Simon L.
A1  - Böhmer, Maik
T1  - Retrograde analysis of calcium signaling by CaMPARI2 shows cytosolic calcium in chondrocytes is unaffected by parabolic flights
T2  - Biomedicines
N2  - Calcium (Ca2+) elevation is an essential secondary messenger in many cellular processes, including disease progression and adaptation to external stimuli, e.g., gravitational load. Therefore, mapping and quantifying Ca2+ signaling with a high spatiotemporal resolution is a key challenge. However, particularly on microgravity platforms, experiment time is limited, allowing only a small number of replicates. Furthermore, experiment hardware is exposed to changes in gravity levels, causing experimental artifacts unless appropriately controlled. We introduce a new experimental setup based on the fluorescent Ca2+ reporter CaMPARI2, onboard LED arrays, and subsequent microscopic analysis on the ground. This setup allows for higher throughput and accuracy due to its retrograde nature. The excellent performance of CaMPARI2 was demonstrated with human chondrocytes during the 75th ESA parabolic flight campaign. CaMPARI2 revealed a strong Ca2+ response triggered by histamine but was not affected by the alternating gravitational load of a parabolic flight.
KW  - CaMPARI
KW  - articular chondrocytes
KW  - cytosolic free calcium
KW  - gravity
KW  - parabolic flight
KW  - high throughput screening
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82179
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-821795
SN  - 2227-9059
N1  - The quantitative imaging data and software codes (release 1.0) presented in this study are openly available in https://github.com/ZeroG-lab/PFCaMPARI at doi:10.5281/zenodo.5824491. Raw images are available on request from the corresponding author (M.B.).
VL  - 10
IS  - 1, art. 138
SP  - 1
EP  - 21
PB  - MDPI
CY  - Basel
ER  -