TY  - INPR
A1  - Lamm, Gerrit H. U.
A1  - Marin, Egor
A1  - Schellbach, Anna V.
A1  - Stetsenko, Artem
A1  - Alekseev, Alexey
A1  - Bourenkov, Gleb
A1  - Borshchevskiy, Valentin
A1  - Asido, Marvin
A1  - Agthe, Michael
A1  - Engilberge, Sylvain
A1  - Rose, Samuel L.
A1  - Caramello, Nicolas
A1  - Royant, Antoine
A1  - Schneider, Thomas R.
A1  - Bateman, Alex
A1  - Mager, Thomas
A1  - Moser, Tobias
A1  - Wachtveitl, Josef
A1  - Guskov, Albert
A1  - Kovalev, Kirill
T1  - CryoRhodopsins: a comprehensive characterization of a new clade of microbial rhodopsins from cold environments
T2  - bioRxiv
N2  - Microbial rhodopsins are omnipresent on Earth, however the vast majority of them remain uncharacterized. Here we describe a new rhodopsin clade from cold-adapted organisms and cold environments, such as glaciers, denoted as CryoRhodopsins (CryoRs). Our data suggest that CryoRs have photosensory activity. A distinguishing feature of the clade is the presence of a buried arginine residue close to the cytoplasmic face of its members. Combining single-particle cryo-electron microscopy and X-ray crystallography with the rhodopsin activation by light, we demonstrate that the arginine stabilizes a strongly blue-shifted intermediate of an extremely slow CryoRhodopsin photocycle. Together with extensive spectroscopic characterization, our investigations on CryoR1 and CryoR2 proteins reveal mechanisms of photoswitching in the newly identified clade and demonstrate principles of the adaptation of these rhodopsins to low temperatures.
Y1  - 2024
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82127
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-821278
UR  - https://www.biorxiv.org/content/10.1101/2024.01.15.575777v1
IS  - 2024.01.15.575777 Version 1
PB  - bioRxiv
ER  -