TY  - JOUR
A1  - Oberoi-Khanuja, Tripat Kaur
A1  - Karreman, Christiaan
A1  - Larisch, Sarit
A1  - Rapp, Ulf Rüdiger
A1  - Rajalingam, Krishnaraj
T1  - Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration
T2  - Journal of biological chemistry
N2  - Inhibitor of apoptosis (IAPs) proteins are characterized by the presence of evolutionarily conserved baculoviral inhibitor of apoptosis repeat (BIR) domains, predominantly known for their role in inhibiting caspases and, thereby, apoptosis. We have shown previously that multi-BIR domain-containing IAPs, cellular IAPs, and X-linked IAP can control tumor cell migration by directly regulating the protein stability of C-RAF kinase. Here, we extend our observations to a single BIR domain containing IAP family member melanoma-IAP (ML-IAP). We show that ML-IAP can directly bind to C-RAF and that ML-IAP depletion leads to an increase in C-RAF protein levels, MAPK activation, and cell migration in melanoma cells. Thus, our results unveil a thus far unknown role for ML-IAP in controlling C-RAF stability and cell migration.
KW  - Cell Migration
KW  - MAP Kinases (MAPKs)
KW  - Molecular Chaperone
KW  - RAF
KW  - Ubiquitination
KW  - XIAP
KW  - Chaperone
KW  - IAP-IAP
KW  - ML-IAP
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76735
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-767355
SN  - 0021-9258
VL  - 287.2012
IS  - 34
SP  - 28445
EP  - 28455
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -