TY  - JOUR
A1  - Diederichs, Tim
A1  - Pugh, Genevieve
A1  - Dorey, Adam
A1  - Xing, Yongzheng
A1  - Burns, Jonathan R.
A1  - Nguyen, Quoc Hung
A1  - Tornow, Marc
A1  - Tampé, Robert
A1  - Howorka, Stefan
T1  - Synthetic protein-conductive membrane nanopores built with DNA
T2  - Nature Communications
N2  - Nanopores are key in portable sequencing and research given their ability to transport elongated DNA or small bioactive molecules through narrow transmembrane channels. Transport of folded proteins could lead to similar scientific and technological benefits. Yet this has not been realised due to the shortage of wide and structurally defined natural pores. Here we report that a synthetic nanopore designed via DNA nanotechnology can accommodate folded proteins. Transport of fluorescent proteins through single pores is kinetically analysed using massively parallel optical readout with transparent silicon-on-insulator cavity chips vs. electrical recordings to reveal an at least 20-fold higher speed for the electrically driven movement. Pores nevertheless allow a high diffusive flux of more than 66 molecules per second that can also be directed beyond equillibria. The pores may be exploited to sense diagnostically relevant proteins with portable analysis technology, to create molecular gates for drug delivery, or to build synthetic cells.
KW  - Biophysical chemistry
KW  - DNA nanostructures
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/53081
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-530817
SN  - 2041-1723
N1  - Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
VL  - 10
IS  - 1, Art. 5018
SP  - 1
EP  - 11
PB  - Nature Publishing Group UK
CY  - [London]
ER  -